Generation of Functional Genetic Study Models in Zebrafish Using CRISPR-Cas9

Carmona-Aldana, F., Nuñez-Martinez, H. N., Peralta-Alvarez, C. A., Tapia-Urzua, G., & Recillas-Targa, F. (2020). Generation of Functional Genetic Study Models in Zebrafish Using CRISPR-Cas9. Cancer Cell Signaling, 255?262. doi:10.1007/978-1-0716-0759-6_16

ABSTRACT

© 2021, Springer Science+Business Media, LLC, part of Springer Nature. CRISPR-Cas9 is a method for genome editing that can be used efficiently for in vivo applications; the basic implementation of this method is used to generate genome site-directed sequence eliminations. Here we describe a protocol for genome editing using CRISPR-Cas9 in zebrafish (Danio rerio) one-cell embryos.



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